Optimization of Explant and Plant Growth Regulators to Increase the Invitro Regeneration Frequency of Potato (Solanum tuberosum L.) cv. SH-5

Author(s) : Muhammad Azher Qureeshi, Iftikhar Ahmad Khan, Bushra Sadia

Abstract:

Efficient in vitro culture system was standardized for potato (Solanum tuberosum) cv. SH-5. Callus formation and plant regeneration were obtained by culture of potato leaf discs and internodal stem segments on MS medium supplemented with different growth regulators. MS medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D) at 4 mg L-1 showed the best results for callogenesis. There was 96% callus induction at 4 mg L-1 of 2,4-D by ,leaf explants as compared to 74% exhibited by intermodal stem segments. Embryogenic calli regenerated shoots on MS supplemented with 2 mg L-1 BAP + 2.5 mg L-1 NAA, had the highest efficiency (64% and 35%) and highest number of shoots per callus (5 and 2) from leaf and stem explants, respectively. Regenerated shoots were separated and rooted on MS medium. The highest rooting response (96%) was observed in the shoots originating from leaf callus as compared to 70% in shoots derived from stem callus. Rooted shoots were acclimatized in the glasshouse for plantation in the field.