Detection of basmati rice using microsatellite markers
Author(s) : Safeena Inam, Syed Adeel Zafar , Malik Attique-ur-Rehman, Muhammad Kashif, Naeem, Nazia Rehman and Muhammad Ramzan Khan*
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Basmati rice is a fine and aromatic rice grown mainly in certain areas of Punjab province in Pakistan. It has high demand in the international market and a source of earning foreign exchange via export. Adulteration of non-basmati rice grains is a major challenge to secure its export standards. Hence, the development of a simple and cost-effective method is necessary to screen the basmati and non-basmati rice samples. In this study, we have validated the efficiency of different molecular markers by screening seven unknown rice samples. Our results demonstrated that three markers namely RM1, RM19 and RM225 proved to be efficient microsatellite molecular markers that could be used to screen basmati and non-basmati rice samples. Further, these results are validated based on expression pattern of Badh2 gene among the basmati and non-basmati rice. Thus, this study provides a contribution towards development of a simple and cost-effective method for rapid screening of basmati rice.