Role of CRISPR/CAS in Agriculture: A brief review

Author(s) : Numra Shehzadi, Afadia Omer, Dua Noor, Junaid Iqbal, Shahid Nazir, Sajid ur Rahman

Digital object identifier:

https://doi.org/10.52587/JAF050103

Abstract:

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) with Cas9 systems have proven to be an effective molecular tool for editing the genome in a variety of organisms, including plants. This technology facilitates to introduce desired mutations at precise location within genomes. In addition, this system also eliminates the usage of transgene and vector free editing with desirable modifications. This tool is being successfully used for the development of disease resistant and climate resilient plant genotypes. This technology not only improves the crop production, but also help in understanding the functions of genes linked with different traits. Hence, CRISPR-Cas9 can be a potential source of second green revolution in the field of agriculture. This review mainly focused on different types and sub-types of CRISPR-Cas system, its classifications and successful application in the field of agriculture to develop disease resistant, stress tolerant and to improve nutritional quality in plants.